Advantages of Whole Genome Amplification (WGA)
PacGenomics’ scientific team has incorporated WGA methods that yield the highest genome coverage with the lowest noise levels subsequent to testing. After conducting a comparison of available methods, we selected and optimized a method that yields greater than 90% genome coverage. This vast coverage allows our platform the greatest chance at detecting smaller copy number variants (CNVs) and segmental aneuploidies across the genome.
As a comparison, the genome coverage of other PGT laboratories is 30-40%. In fact, some labs use targeted sequencing methods, which provide far less than 30% genome coverage.
Library Preparation and Automation
PacGenomics utilizes multiple robotic systems to perform library preparation and normalizations. This reduces the chance of human error and allows us to produce consistent and reliable results. Fully automated library preparation also works to reduce bench work time and in turn, reduce cost.
DNA Whole-Genome Sequencing
PacGenomics NGS-based PGT platform generates a higher number of reads per sample and has a detection limit of 3.5Mb, the most sensitive PGT platform on the market. This allows for higher sensitivity in the detection of chromosomal abnormalities across greater amounts of the genome. In fact, due to the sensitivity and resolution of our platform, we have been able to detect evidence in embryo biopsy samples of a balanced translocation in many patients whose carrier status was otherwise unknown.
As a comparison, other PGT laboratories’ platforms have detection limits ranging from 5-20Mb.
